Despite these research efforts on reproduction, no attempts have been made to experimentally address the effect of density on mate selection in copepods.
In this investigation, we video recorded the behaviour of the free-swimming Calanoid copepod (Dana) at different densities to test the hypothesis that density affects mate selection.
However, states of copepods such as age (Ceballos and Kiørboe, 2011), sexual status (Heuschele and Kiørboe, 2012) and size (Ceballos and Kiørboe, 2010) have been reported to affect mate selection.
Therefore, mating durations and proportions of mating attempts followed by a resting phase (successful courtship swimming behaviours) were used to assess changes in female mate choice among treatments.Our observations showed that the number of mating events is consistently lower than the encounter rate.In fact, only 1% of potential (theoretical) encounters led to mating attempts.To date, it is not known whether males without a spermatophore continue searching for females or not. Nevertheless, spermatophore production in (Bagøien and Kiørboe, 2005).
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The availability of individuals from the opposite sex can result from a change in animal density.In fact, density can have strong effects on sexual selection (Kokko and Rankin, 2006) which in turn could affect females' fecundity.After their sex was identified, they were placed in the observation aquarium and allowed to acclimate for A mating attempt was defined as an irregular swimming behaviour of a pair of copepods (i.e.courtship swimming behaviour) followed, or not, by a resting phase during which the male copepod could transfer the spermatophore to the female.) for which their egg production is known to be affected.
The ratio of observed mating attempts to theoretical encounters was constant among treatments.The total number of observed mating attempts for a given density = 0.21).Experimental studies of mating in copepods are generally performed using males with a spermatophore and virgin females.In calanoid copepods, density affects reproduction in different ways.First, both negative effects (Peck and Holste, 2006) and no effects (Jepsen (Ban and Minoda, 1994).